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rraadd_8
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@rraadd_8-11857
Last seen 4.5 years ago

Dear users,

Instead of counts (non-negative integers) required by DeSeq2, I have negative numbers and fractions,

and I need to use DeSeq's statistics to find out significantly up/down genes.

Since for my data, min=-10 and max=10, I can convert it into counts by

- first multiplying with a constant eg. 1000 to preserve the decimal precision.
- and then add some constant value eg. 10000 to make everything positive.

Would it hamper my analysis?

Thanks. I wonder if it is possible, (independent of DeSeq2) to use Wald test for negative numbers?